Core Facilites and Core Services

At the beginning of 2016, a “core” structure was put into effect that organized facility and service units as independent organizational entities from FLI’s research groups. A number of technology platforms (e.g. sequencing, mass spectrometry) grew out of individual methodological requirements for single research groups in the last years but developed into semiautonomous substructures. As consequence of re-focused research activities and the concomitant advent of new research groups at FLI, those units increasingly had to serve many FLI groups and collaborative research efforts in the Jena research area.

To accommodate this development and to increase efficiency as well as transparency for users, facility personnel and for administrative processes, it came natural to re-organize such activities into independent units as “FLI Core Facilities and Services” and to phase out infrastructures considered non-essential for FLI’s research focus (X-ray crystallography and NMR spectroscopy).

FLI’s Core Facilities (CF) are managed by a CF Manager and are each scientifically guided in their activities and development by an FLI Group Leader, as Scientific Supervisor. The animal facilities comprising fish, mouse and transgenesis are run separately, as they involve a more complex organizational structure. Basic Core Services (CS) are directly led by the Head of Core (HC), who in turn is supported by individual CS Managers.

All facilities and services, including animal facilities, have a valuable contribution to FLI’s research articles; e.g. from 2016–2018, to 54% of all peer reviewed research publications.

Overview Core Facilities and Core Services at FLI.

Publications

(since 2016)

2018

Cell-based RNAi screening and high-content analysis in primary calvarian osteoblasts applied to identification of osteoblast differentiation regulators.
Ahmad M, Kroll T, Jakob J, Rauch A, Ploubidou A, Tuckermann J
Sci Rep 2018, 8(1), 14045
A new RelB-dependent CD117+ CD172a+ murine dendritic cell subset preferentially induces Th2 differentiation and supports airway hyperresponses in vivo.
Andreas N, Riemann M, Castro CN, Groth M, Koliesnik I, Engelmann C, Sparwasser T, Kamradt T, Haenold R, Weih F
Eur J Immunol 2018, 48(6), 923-36
Transcriptomic alterations during ageing reflect the shift from cancer to degenerative diseases in the elderly.
Aramillo Irizar P, Schäuble S, Esser D, Groth M, Frahm C, Priebe S, Baumgart M, Hartmann N, Marthandan S, Menzel U, Müller J, Schmidt S, Ast V, Caliebe A, König R, Krawczak M, Ristow M, Schuster S, Cellerino A, Diekmann S, Englert C, Hemmerich P, Sühnel J, Guthke R, Witte OW, Platzer M, Ruppin E, Kaleta C
Nat Commun 2018, 9(1), 327
Naked mole-rat transcriptome signatures of socially suppressed sexual maturation and links of reproduction to aging.
Bens* M, Szafranski* K, Holtze S, Sahm A, Groth M, Kestler HA, Hildebrandt** TB, Platzer** M
BMC Biol 2018, 16(1), 77 * equal contribution, ** co-senior authors
Spatial tissue proteomics quantifies inter- and intra-tumor heterogeneity in hepatocellular carcinoma.
Buczak* K, Ori* A, Kirkpatrick JM, Holzer K, Dauch D, Roessler S, Endris V, Lasitschka F, Parca L, Schmidt A, Zender L, Schirmacher P, Krijgsveld J, Singer** S, Beck** M
Mol Cell Proteomics 2018, 17(4), 810-25 * equal contribution, ** co-corresponding authors
On the S-layer of Thermus thermophilus and the assembling of its main protein SlpA.
Farci D, Farci SF, Esposito F, Tramontano E, Kirkpatrick J, Piano D
Biochim Biophys Acta 2018, 1860(8), 1554-62
Integrative analysis of differentially expressed genes and miRNAs predicts complex T3-mediated protective circuits in a rat model of cardiac ischemia reperfusion.
Forini F, Nicolini G, Kusmic C, D'Aurizio R, Rizzo M, Baumgart M, Groth M, Doccini S, Iervasi G, Pitto L
Sci Rep 2018, 8(1), 13870
The eukaryotic linear motif resource - 2018 update.
Gouw M, Michael S, Sámano-Sánchez H, Kumar M, Zeke A, Lang B, Bely B, Chemes LB, Davey NE, Deng Z, Diella F, Gürth CM, Huber AK, Kleinsorg S, Schlegel LS, Palopoli N, Roey KV, Altenberg B, Reményi A, Dinkel H, Gibson TJ
Nucleic Acids Res 2018, 46(D1), D428-34
Molecular mechanisms of photoadaptation of photosystem I supercomplex of in an evolutionary cyanobacterial/algal intermediate.
Haniewicz P, Abram M, Nosek L, Kirkpatrick J, El-Mohsnawy E, Janna Olmos JD, Kouril R, Kargul JM
Plant Physiol 2018, 176(2), 1433-51
Conformational µ-conotoxin PIIIA isomers revisited: The impact of cysteine pairing on disulfide bond assignment and structure elucidation.
Heimer P, Tietze AA, Bäuml CA, Resemann A, Mayer FJ, Suckau D, Ohlenschläger O, Tietze D, Imhof D
Anal Chem 2018, 90(5), 3321-7