Methodology

To study YAP/TAZ-mediated transcription and its phenotypes in vivo, state-of-the-art methods are used:

• Single cell technologies: scRNA-Seq, CITE-Seq, scATAC-Seq
• Pooled in vivo CRISPR screens in combination with single cell transcriptomics: CROP-Seq, Perturb-Seq
• Genome-wide transcriptomics: CUT & RUN, CUT & TAG, ATAC-Seq, RNA-Seq, 4SU-Seq, SLAM-Seq
• Pooled genome-wide CRISPR screens and focused screens: CRISPR, shRNA, SAM and siRNA.
• Inducible mouse models