CF Flow Cytometry (FACS)

The facility carries out sorting and flow cytometric analysis of cells and is available to FLI researchers and external users upon arrangement. In addition, the facility provides the instrumentation for sorting small living organisms (e.g. worms, small fishes) based on an optical sorting criterion.

The instrumentation includes:

  • five cell sorters (one in S2 lab)
  • five analyzers
  • one BioSorter.

The facility staff supports users with:

  • the planning and setting up of experiments prior to flow cytometric analysis or sorting
  • troubleshooting of technical problems
  • complete data analysis or assistance during analysis of complex data sets
  • introduction into the basics of flow cytometric analysis and operating of the machines.

Sorting of

  • HSC populations/embryonic stem cells
  • muscle satellite cells
  • interneurons and neuronal reporter cells
  • podocytes
  • mouse B lymphocytes, T lymphocytes and dendritic cells from lymphoid and non-lymphoid tissues
  • GFP-positive cells to maintain genetically modified cells.

Analysis of

  • conventional cell stainings (surface, intra-cellular, intra-nuclear)
  • DNA content vs. BrdU/EdU
  • Annexin V vs. DNA-intercalating dyes
  • fluorescent cell signaling mutants
  • enzyme complexes via Fluorescent Resonance Energy Transfer (FRET).

Internal users:
Additional information on sorters and analyzers.

External users:
Please inquire.

Team (incomplete due to Data protection)

Name Phone Email Position
Katrin Schubert +49 3641 656022/6758 katrin.schubert@leibniz-fli.de Core Facility Manager
Linda Rothenburger +49 3641 656762/6758 linda.rothenburger@leibniz-fli.de Research Engineer
Michelle Antoinette Burkhardt +49 3641 656749 michelle.burkhardt@leibniz-fli.de Technical Assistant
Björn von Eyß +49 3641 656047 bjoern.voneyss@leibniz-fli.de Scientific Supervisor

* incomplete due to Data protection

For publications of individual CF members:  publications search 

Contact

Dr. Katrin Schubert

Katrin Schubert
CF Manager
+49 3641 65-6758 or -6022
katrin.schubert@leibniz-fli.de

Björn von Eyß
Scientific Supervisor
bjoern.voneyss@leibniz-fli.de

Group Website

Overview CF Flow Cytometry

Overview CF Flow Cytometry
Overview CF Flow Cytometry