CF Next Generation Sequencing
The CF Next Generation Sequencing provides FLI researchers and external collaborators with comprehensive expertise and state-of-the-art infrastructure in NGS technologies. Our goal is to support researchers in addressing biological questions at the genomic, transcriptomic, epigenomic, and metagenomic level with the high precision and efficiency.
We support projects throughout the entire experimental pipeline – from consultation on experimental design and sample requirements through library preparation and sequencing to standardized primary data processing. Downstream analyses are performed in cooperation with the CF Life Science Computing and are not part CF NGS core services.
Applications
- Genomics – whole-genome, exome, and targeted sequencing
- Transcriptomics – bulk RNA-seq, single-cell RNA-seq, small RNA
- Epigenomics – methylation analyses (RRBS, WGBS), ATAC-seq
- Metagenomics and microbiome
- Long-read sequencing
Equipment
Sequencing
- Illumina NovaSeq 6000, NextSeq 1000, MiSeq
- Oxford Nanopore MinION Mk1B
Sample preparation and automation
- 10x Genomics Chromium X
- Beckman Coulter Biomek i7
- Covaris M220
- Diagenode Megaruptor
- Sage Science BluePippin
Quality control
- Agilent 4200 TapeStation, 2100 Bioanalyzer
- Thermo Scientific NanoDrop
