CF Imaging
The Core Facility Imaging provides the infrastructure to support state-of-the-art microscopy at the FLI. Currently, the Facility operates and maintains several widefield and confocal microscopes, a multi-slide scanner, a laser microdissection/optical tweezer, a Lightsheet and a STED super resolution microscope.
The facility staff provides introduction into microscopy techniques and service for FLI researchers and, upon arrangement, also for external users.
The Facility is specialized in live cell microscopy to monitor molecule and cellular dynamics in space and time. Several fluorescence fluctuation microscopy (FFM) techniques with single-molecule resolution have been established including FRAP, FCS, FCCS, FLIM, FRET, and RICS.
Equipment and techniques available:
LSM/ConfoCor3 (FRET, FRAP, FCS, FCCS, RICS and time-lapse using APDs)
Airyscan I (multi-fluorescence, rapid live-cell imaging, super resolution)
Airyscan II (multi-fluorescence, rapid live-cell imaging, super resolution, Dynamics Profiler, Spectral RICS)PALM Microdissection/Optical Tweezer
PALM Microdissection/Optical Tweeze
Apotome optical sectioning (Widefield, brightfield, colocalization analysis, 2D, 3D, distance measurements, tiling of tissue sections)
Multi-Slide Scanner
TCS SP8 STED-3X/FALCON (STED: Stimulated emission depletion super-resolution microscopy; FALCON: fast lifetime contrast)
Lightsheet Z1 (live imaging of whole organisms in 3D over time).
Image quantitation (Huygens, Imaris, Arivis, Metamorph)
FLI's Imaging Facility is part of the German BioImaging Network (GERBI).
