CF DNA Sequencing

The Facility provides next-generation DNA sequencing (NGS) technology to the FLI. External users may also have access to the technology, e.g. upon collaborative arrangements. In addition to sample DNA/RNA quality check, library preparation and sequencing, the facility provides first-pass data processing, data quality check and analysis. A number of pipelines for deeper data analysis are established and available, also via the CF Life Science Computing of the FLI.

Beyond analyzing whole genomes or subfractions thereof (e.g. exomes), NGS is ideally suited to provide insight into fundamental biological processes, such as transcription, translation, DNA methylation, histone modification, protein-nucleic acid interaction and much more.

The Facility operates the following technology platforms:

  • Illumina HiSeq2500
  • Illumina NextSeq500
  • Illumina MiSeq
  • PacBio RSII
  • Fluidigm C1 device for single-cell polyA+-RNA/DNA preparation
  • 10x Chromium Controller single-cell preparation.
Set-up of a sequencing run
FLI: Set-up of a sequencing run
  • RNA-seq: sequencing of polyA+/rRNA-RNA for analysis of differentially expressed genes or transcriptome assembly
  • SmallRNA-seq: sequencing of small/micro RNAs for expression profiling
  • ISO-seq: sequencing of polyA+RNA for transcriptome assembly/transcript isoform discovery
  • ART-seq: sequencing of ribosome-bound RNA for translation analysis
  • ChIP-seq: sequencing of DNA following chromatin immunoprecipitation to identify protein binding sites
  • WES: whole exome sequencing for variant discovery
  • WGS: whole genome sequencing for variant discovery or genome assembly
  • Amplicon-seq: amplicon sequencing for variant discovery or DNA methylation analysis
  • WGBS/RRBS: whole genome/reduced representation bisulfite sequencing for DNA methylation analysis (5mC and/or 5hmC)

Internal users:
Download (excel) Sample form template for requesting NGS.

External users:
Please inquire.

Team (incomplete due to Data protection)

Name Telefon E-Mail Tätigkeit
Marco Groth +49 3641 656054 marco.groth@leibniz-fli.de Core Facility Manager
Martin Bens +49 3641 656801 martin.bens@leibniz-fli.de Postdoc
Ivonne Görlich +49 3641 656242 ivonne.goerlich@leibniz-fli.de Technischer Assistent
Cornelia Luge +49 3641 656442 cornelia.luge@leibniz-fli.de Technischer Assistent
Christoph Englert +49 3641 656042 christoph.englert@leibniz-fli.de Wissenschaftlicher Betreuer

* unvollständig aufgrund DSGVO

For publications of individual CF members:  publications search 

Contact

Dr. Marco Groth

Marco Groth
CF Manager
+49 3641 65-6054
marco.groth@leibniz-fli.de

Christoph Englert
Scientific Supervisor
christoph.englert@leibniz-fli.de

Group Website

Overview CF DNA Sequencing

Overview CF DNA Sequencing
Overview CF DNA Sequencing