CS Histology, Pathology & Electron Microscopy

The Core Service provides services to FLI researchers for standard histological techniques, including processing of tissues, sectioning and preparation of samples for the EM. EM service for external users is available upon arrangement.

Some standard histological stainings are also available, carried out either by the CS staff or by users themselves with guidance provided by the CS staff where appropriate.

Pathology assessment of sections is supported by an experienced pathologist, who may be contacted in advance directly if help is desired.

The major services include paraffin sections, cryo-sections and histological stainings (H&E, Masson-Trichrome, NADH, Sirius Red, COX, Sudan-3).

The CS maintains the following equipment:

  • Tissue processor
  • Embedding center
  • 2 Microtomes
  • Ultramicrotome for EM
  • 3 Cryostats
  • Automatic stainer for H&E staining
  • Cover slipper
  • Transmission electron microscope
  • Some secondary antibodies (fluorescent and Peroxidase), available upon request
Mouse kidney: Masson trichrome staining
FLI: Mouse kidney
Microvilli of an N. furzeri proximal renal tubule (bar = 1µm)
FLI: Microvilli of an N. furzeri proximal renal tubule

Contact

Dr. Julia von Maltzahn

Julia von Maltzahn
CS Manager
+49 3641 65-6720
julia.vonmaltzahn@leibniz-fli.de

Group Website

Pathology:
Please make arrangements with Lucien Frappart (Lucien.frappart@leibniz-fli.de) directly in advance.

Sections:
Contact Maik Baldauf (maik.baldauf@leibniz-fli.de) or Sabine Matz (sabine.matz@leibniz-fli.de)

Stainings:
For H&E contact Maik Baldauf (maik.baldauf@leibniz-fli.de), for all other stainings Sabine Matz (sabine.matz@leibniz-fli.de)

Electron microscopy:
Contact either Julia von Maltzahn (julia.vonmaltzahn@leibniz-fli.de) or Katrin Buder (katrin.buder@leibniz-fli.de).

Antibodies available:
Contact Katrin Buder (katrin.buder@leibniz-fli.de) for a list of currently available secondary antibodies and aliquots.

Internal users:
 Download (pdf)  Guideline for Histology Users

External users:
Please inquire with Julia von Maltzahn.

Team

Name Phone Email Position
Julia von Maltzahn +49 3641 656720 julia.vonmaltzahn@leibniz-fli.de Core Service Manager
Katrin Buder +49 3641 656173 katrin.buder@leibniz-fli.de Technical Assistant
Sabine Matz +49 3641 656301 sabine.matz@leibniz-fli.de Technical Assistant
Linda Rothenburger +49 3641 656033 linda.rothenburger@leibniz-fli.de Technical Assistant
Lucien Frappart +49 3641 656031 lucien.frappart@leibniz-fli.de Consultant

For publications of individual CS members:  publications search