CF Flow Cytometry (FACS)

The facility carries out sorting and flow cytometric analysis of cells and is available to FLI researchers and external users upon arrangement. In addition, the facility provides the instrumentation for sorting small living organisms (e.g. worms, small fishes) based on an optical sorting criterion.

The instrumentation includes:

  • four cell sorters
  • four analyzers
  • one BioSorter

The facility staff supports users with:

  • the planning and setting up of experiments prior to flow cytometric analysis or sorting
  • troubleshooting of technical problems
  • complete data analysis or assistance during analysis of complex data sets
  • introduction into the basics of flow cytometric analysis and operating of the machines

Contact

Katrin Schubert
CF Manager
+49 3641 65-6758 or -6704
katrin.schubert@leibniz-fli.de

Björn von Eyß
Scientific Supervisor
bjoern.voneyss@leibniz-fli.de

Group Website

Overview CF Flow Cytometry

Overview CF Flow Cytometry

Sorting of

  • HSC populations/embryonic stem cells
  • muscle satellite cells
  • interneurons and neuronal reporter cells
  • podocytes
  • mouse B lymphocytes, T lymphocytes and dendritic cells from lymphoid and non-lymphoid tissues
  • GFP-positive cells to maintain genetically modified cells

Analysis of

  • conventional cell stainings (surface, intra-cellular, intra-nuclear)
  • DNA content vs. BrdU/EdU
  • Annexin V vs. DNA-intercalating dyes
  • fluorescent cell signaling mutants
  • enzyme complexes via Fluorescent Resonance Energy Transfer (FRET)

Internal users:
  Download (pdf)  Additional information on sorters and analyzers

External users:
Please inquire.

Team

Name Phone Email Position
Katrin Schubert +49 3641 656758/6704 katrin.schubert@leibniz-fli.de Core Facility Manager
Maria Locke +49 3641 656762 maria.locke@leibniz-fli.de Research Engineer
Sebastian Benkhoff +49 3641 656844 sebastian.benkhoff@leibniz-fli.de Technical Assistant
Björn von Eyß +49 3641 656047 bjoern.voneyss@leibniz-fli.de Scientific Supervisor

For publications of individual CF members:  publications search